LOCUS       40924_48218        4641 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pYMD1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Binding sequences for RdgB, a DNA damage-responsive transcriptional 
            activator, and temperature-dependent expression of bacteriocin and 
            pectin lyase genes in Pectobacterium carotovorum subsp. carotovorum
  JOURNAL   Appl. Environ. Microbiol. 74 (19), 6017-6025 (2008)
  PUBMED    18689515
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2007) Contact:Kazuteru Yamada Graduate School of 
            Agricultural Science, Tohoku University, Laboratory of Applied 
            Microbiology, Department of Microbial Biotechnology; 1-1 
            Tsutsumi-dori Amaiya-machi, Aobaku, Sendai 981-8555, Japan
REFERENCE   3  (bases 1 to 4641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "19"; 
            pages: "6017-6025"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2007) Contact:Kazuteru Yamada Graduate School of 
            Agricultural Science, Tohoku University, Laboratory of Applied 
            Microbiology, Department of Microbial Biotechnology; 1-1 
            Tsutsumi-dori Amaiya-machi, Aobaku, Sendai 981-8555, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        110..555
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             556..1974
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(2708..2724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2760..3143)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3652..4509)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4510..4614)
                     /label=AmpR promoter
ORIGIN
        1 atcgcaggaa acagctatga ccatgattac gaattcgagc tcggtgcccg gggatcctct
       61 agagtcgacc tgcaggtcga ctctagagga tcgatccttt ttaacccatc acatatacct
      121 gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt gtgattaaaa
      181 aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga aaagaaacag
      241 atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa
      301 caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa
      361 agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt gtaaagggca
      421 aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt gtgcgtaact
      481 aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca gtacataaaa
      541 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
      601 actaccgcac tgctggcagg aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag
      661 ccatataagg aaacatacgg catttcccat attacacgcc atgatatgct gcaaatccct
      721 gaacagcaaa aaaatgaaaa atatcaagtt cctgaattcg attcgtccac aattaaaaat
      781 atctcttctg caaaaggcct ggacgtttgg gacagctggc cattacaaaa cgctgacggc
      841 actgtcgcaa actatcacgg ctaccacatc gtctttgcat tagccggaga tcctaaaaat
      901 gcggatgaca catcgattta catgttctat caaaaagtcg gcgaaacttc tattgacagc
      961 tggaaaaacg ctggccgcgt ctttaaagac agcgacaaat tcgatgcaaa tgattctatc
     1021 ctaaaagacc aaacacaaga atggtcaggt tcagccacat ttacatctga cggaaaaatc
     1081 cgtttattct acactgattt ctccggtaaa cattacggca aacaaacact gacaactgca
     1141 caagttaacg tatcagcatc agacagctct ttgaacatca acggtgtaga ggattataaa
     1201 tcaatctttg acggtgacgg aaaaacgtat caaaatgtac agcagttcat cgatgaaggc
     1261 aactacagct caggcgacaa ccatacgctg agagatcctc actacgtaga agataaaggc
     1321 cacaaatact tagtatttga agcaaacact ggaactgaag atggctacca aggcgaagaa
     1381 tctttattta acaaagcata ctatggcaaa agcacatcat tcttccgtca agaaagtcaa
     1441 aaacttctgc aaagcgataa aaaacgcacg gctgagttag caaacggcgc tctcggtatg
     1501 attgagctaa acgatgatta cacactgaaa aaagtgatga aaccgctgat tgcatctaac
     1561 acagtaacag atgaaattga acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg
     1621 ttcactgact cccgcggatc aaaaatgacg attgacggca ttacgtctaa cgatatttac
     1681 atgcttggtt atgtttctaa ttctttaact ggcccataca agccgctgaa caaaactggc
     1741 cttgtgttaa aaatggatct tgatcctaac gatgtaacct ttacttactc acacttcgct
     1801 gtacctcaag cgaaaggaaa caatgtcgtg attacaagct atatgacaaa cagaggattc
     1861 tacgcagaca aacaatcaac gtttgcgcca agcttcctgc tgaacatcaa aggcaagaaa
     1921 acatctgttg tcaaagacag catccttgaa caaggacaat taacagttaa caaataaaaa
     1981 cgcaaaagaa aatgccgata tcctattggc attttctttt atttcttatc aacataaagg
     2041 tgaatcccat atgaactata taaaagcagg caaatggcta accgtattcc taaccttttg
     2101 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
     2161 tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc caggtgctgc
     2221 ctcagattca ggttatgccg ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc
     2281 tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatat caccacgtca
     2341 aagggtgaca gcaggctcat aagacgcccc agcgtcgcca tagtgcgttc accgaatacg
     2401 tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa acagccagcg ctggcgcgat
     2461 ttagccccga catagcccca ctgttcgtcc atttccgcgc agacgatgac gtcactgccc
     2521 ggctgtatgc gcgaggttac cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt
     2581 tgaggccaac gcccataatg cgggctgttg cccggcatcc aacgccattc atggccatat
     2641 caatgatttt ctggtgcgta ccgggttgag aagcggtgta agtgaactgc aggcatgcaa
     2701 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccatggc taattcccat
     2761 gtcagccgtt aagtgttcct gtgtcactga aaattgcttt gagaggctct aagggcttct
     2821 cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc
     2881 cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga
     2941 tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt
     3001 acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag
     3061 cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc
     3121 aacaggttga actgcggatc ttgcggccgc aatcgggcaa atcgctgaat attccttttg
     3181 tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc
     3241 acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca
     3301 aggaaactca aaataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg
     3361 ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt
     3421 ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag
     3481 taaggcagcg gtatcatcaa cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3601 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3661 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3721 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3781 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3841 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3901 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3961 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4021 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4081 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4141 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4201 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4261 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4321 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4381 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4441 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4501 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4561 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4621 tccccgaaaa gtgccacttg c
//
